Higher density microplates (384- or 1536-well microplates) are typically used for screening applications, when throughput (number of samples per day processed) and assay cost per sample become critical parameters, with. Get Quote . 96-well fluorescent cell dilution series, top-read. 5 pM 2. FLIPR Calcium 4 Assay Kit. x ABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Turn the. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. Made from polypropylene with conical well profiles to. 0 and dispense 10 μL to all wells of the 384-well plate(s) (see Note 13). Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. There is no product in the shopping cart, buy it!. 0. Other technologies such as imaging. 20 plates/hour (2-minute read time) 96-well 1,920 data points/hour 384-well 7,680 data points/hour 1536-well 30,720 data points/hour Bar code manual mode Keyboard entry Bar code remote mode Short or long side Temperature control Ambient +5°C to 40°C General specifications Dimensions (in. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. The VICTOR® Nivo™ system packs all popular detection modes into the industry’s smallest multimode reader footprint. Supplier: Molecular Devices. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. 5 fg per well to 0. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. 6, 12, 24, 48, 96, 384-well plates : ANSI/SLAS Light source. Its software interface is easy to learn and use, and lets you control the instrument from almost any device – perfect for multiuser labs. 76 ± 0. Read one sample or up to 384 in a single plate eead using any standard cuvette, or 96-or 384-well microplate. the use of a reader calibrated with primary NIST and NMI standards. com Check our website for a current listing of worldwide distributors. Instructions for adding new plates: CSI/CP2: Add plate. M2. To prevent evaporation, the microplate was covered with a qPCR seal. $245. M2. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. for the SpectraMax Multi-Mode Microp late Reader, as well as all other Molecular Devices' microplate readers. DynaPro ™ Plate Reader III. Description. 96 Well Plate Reader found in: Well Plate Reader Accessory, SmartReader™ 96 Microplate Absorbance Reader, SmartReader UV-Vis Microplate Reader, Stratus. minutes and begin reading microplates with seven mouse clicks. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. Figure 6. 9. Plate chamber design of the SpectraMax category 1 microplate readers. The perfect microplate reader for everyday biochemical assays, cell-based assays, molecular biology and immunoassays. (Component A, C) is sufficient for 1 plate (96-, 384-, 1536-well). #3-000-510)Page 154: Ordering Parts And Accessories. x. Spectranet customer portalThe microplate reader has been around for more than three decades and continues to serve as a must-have workhorse of clinical, academic research and industrial laboratories alike. If no reference reading is taken, the instrument uses the I 0 values stored in the NVRAM (non-volatile memory) of the. 2/24/2015 Molecular Devices FlexStation 3 Russ Desnoyer Our lab needed a way to measure Calcium flux downstream of. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. Access this by going to. It is also important to note that organoid growth patterns are relatively consistent from 24- to 384-well plates. This reader also makes use of a unique temperature. 1 or 10 Tablet PC with USB connection to instrument: Read Speed: 96-well plate: less than 1 minute; 384-well plate: less than 3 minutes: Automation Compatibility: Hardware and software accessible to third-party hardware/software control, including LIMS. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. FLIPR Calcium 4 Assay Kit (Molecular Devices, San Jose, CA, USA). The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. 0 out of 5 $ 8,495. For more sample throughput, the system canThe SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. These robust readers have been placed in labs from Antarctica to the. The color touch screen provides a visual user interface, making programming fast and intuitive. 3860 N First Street. This kit is designed for customers who. These advertising cookies will no longer be set for this browser and device. 19 H mm that meet microplate industry standards. Plates were read in the SpectraMax M5 microplate reader at 485ex/515em with bottom read. 96- and 384-well microplates. The fluorescent intensity of each well was measured by using a fluorescence plate reader (SpectraMax Paradigm, Molecular Devices, Sunnyvale, CA, USA) at 588-nm excitation and 633-nm emission. #0200-7011) Instrument setup The results above revealed excellent detection limits and dynamic range in all three Molecular Devices microplate luminometers. A thermal cycling reaction was immediately initiated. Industry standard disposable (choose from recommended list). Service Contracts, Preventative Maintenance and. C. A preconfigured protocol with optimized reader settings enabled reading of a 384-well plate in just two minutes. Simultaneous dual emission detection enables read times of only 2:17 for an entire 384-well plate. 100 fmol luciferase per well. Molecular Devices Gemini XS Microplate Spectrofluorometer Details. A ten-fold serial dilution of purified luciferase in PBS with 0. Integrated stacker option. #W50-100). Wash heads are made of Polyethylene Terephthlate (PET) with stainless steel probes to prevent corrosion. Two high-energy xenon flash lamps: Reading capabilities. 96- and 384-well microplates. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. ,LTD (CAT#: STEM-S. Outer wells aligned for proper viewing with FLIPR; Ideal for miniaturized 384 well fluorescent. 96-well clear LAL Reagent Grade™ Multi-well Plates (Lonza cat. Molecular Devices Spectramax. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofThe plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). * Note: Programming the washer with a final aspirate height of 4. Following the flushing of the machine as described in Section 3. 100 fmol 1536-well (8 µL) 0. …200, the Bio-Tek µQuant™, the Bio-Rad UltraMark™, the Molecular Devices SPECTRAmax® 190 and 250 plate readers. FlexStation 3 Multi Mode Microplate Reader. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. , San Diego, CA, USA) such that the. 306 plate air cal. The VICTOR Nivo. 100 µL (96-well assay) or 25 µL (384-well assay) of CellTiter-Glo reagent was added to each well. There is no product in the shopping cart, buy it!. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells containing luciferase and controls. Click SoftMax Pro Software User Guide. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. (1536-well plate) 2 µL (quartz cuvette), 4 µL (disposable cuvette). 384- or 96-well read plate containing cells loaded. 0nm increments. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. That explains the lower values on the LmaxII384 plate reader. -Flex3 link -we don't have this) complements the Tecans, in that it has an internal 8- or 16-tip pipetting head and performs multi-label measurements [fluorescence, fluorescence resonance energy transfer (FRET) and chemiluminescence] in real-time in 96 or 384 well format. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. Total volume (except deep well plates) is 120 mL. Tunable wavelength selection / monochromator. or use the modified protocol below for the generation of NPCs (see Note 1). Danaher Inc 384 well plate 384 Well Plate, supplied by Danaher Inc, used in various techniques. 96-deep-round-well-plate, 384-deep-square-well-plate, 24-deep-well-plates. 384 well plates 6–384 wells 6–384 wells 6–384 wells 6–1,536 wells (fluorometry, TRF, luminometry, AlphaScreen assays). 1 a. x. #4TI-0214) UltraVision Plate 96 (Brooks Life Sciences cat. With the QuickSync client installed on your computer, your SpectraMax iD3 reader can push data to any computer within the same network. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Explore the best practices for running absorbance-based assays how Molecular Devices absorbance readers can be utilized to optimize said absorbance-based assays. Plus 384. use as little as 2 µL samples with 24- or 64-well plates. To update the driver. Whether running fluorescence imaging or brightfield assays, the automated imager features a comprehensive portfolio of preconfigured protocols for cell-based assays to shorten the learning curve, so. –Min. (or plate reader). Industry standard disposable (choose from recommended list). 더 많은. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one compact benchtop reader. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. Results shown are from the SpectraMax M5 microplate reader, but similar results are obtained with other Molecular Devices readers with fluorescence detection mode (data not shown). The Embla 96/384 Well Washer accepts 96-and 384-well format microplates and is ideal. 0112-0127C 9 Chapter1:Introduction TheFlexStation® 3Multi-ModeMicroplateReadercombinestheperformanceofadual-monochromator,multi-modemicroplatereaderandanintegrated8. Ensure data accuracy of your reader. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. Figure 1. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. Outer wells are aligned for proper viewing with FLIPR. FlexStation 3 Benchtop Multi-Mode Microplate Reader User Guide Ordering Parts and Accessories To obtain parts and accessories, contact Molecular Devices Customer Service or your authorized service representative. x. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum well volume needed to run a 384 well plate? Minimum volume. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Overview. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader. Tecan’s quality control and validation packages help our customers to fulfill all regulatory requirements for their Tecan microplate readers in an efficient, cost-effective way. With SoftMax Pro Software, the PathCheck Sensor automatically normalizes the well absorbance. DynaPro ™ Plate Reader III. Temperature control: 4°C above ambient to 45°C. Dynamic range > 6 decades . #6590-0118) Filter 665/10 (Molecular Devices cat. Aspirating and dispensing of wash buffer can occur simultaneously in all wells via AquaMax® 4000 Microplate Washer with a 96-. The FLIPR 384 system comes complete with 384. Fluorescent Imaging Plate Reader (FLIPRTM ) technology to measure Ca2+ flux in cells was an important development in the early 1990’s and has played a significant role in HTS and lead optimization applications. Microplate readers provide the ideal measurement platform for the high-throughput. 384 Well Optically Clear Tissue Culture Plate: Plate length: 127. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. 20 attomol ATP per well. Powerful, benchtop microplate stacker that works seamlessly with Molecular Devices microplate readers and washers. 1. 2. FlexIII. The absorbance was then read on the SpectraMax microplate reader at 426 nm, and the resulting OD values for all wells were averaged together to obtain a value of 0. You should ensure that there is enough volume in compound plate wells to operate the IonWorks® system taking into account the dead volume. Published Dec 16, 2020 | Updated Dec 16, 2020 Products : SpectraMax 340PC 384 (Obsolete), SpectraMax Plus 384 reader What is the minimum amount of DNA needed. The plate reader can measure multiple readouts, including UV and. Fluorescence Microplate Reader Comparison Chart. 1. All stand-alone instrument functions can be controlled using the software. All measurements were performed in six replicates and cell toxicity was calculated as the percentage of the OD value of untreated or treated. VIS absorbance detection. Contact Us Phone: +1-800-635-5577 Web: Email: info@moldev. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. Minimum volume and maximum throughput with 96- or. These models can no longer be repaired. CO 2 & O 2 control. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. for ELISAs and most cell-based assays. Before you do a bottom read, or use high profile (6-well, 12-well, 24-well, or 48-well) plates, you must remove the plate adapter from the plate drawer. 190. Plate Formats: 6- to 384-well plates, NanoQuant Plate (Sold Separately) Detection Modes: Luminescence, Fluorescence, Absorbance. Select Consumables. VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Use the provided 3. x. Biotek Synergy 2 Plate Reader Multi-Mode. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. Scan every well . Absorbance equation. In addition, SoftMax Pro SoftwareBelow is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. Incubate the plates at room temperature for 20 min before reading plate luminescence using a Paradigm plate reader (Molecular Devices) (see Note 14). 96-, 384-, and 1536-well microplates. Published Dec 16, 2020 | Updated Nov 07, 2022. flat meniscus), but is approx 15 mL. Microplate readers are commonly used in life science laboratories. However, for greater flexibility, some instruments can accommodate 6- to 1536-well plate formats. 7. Molecular Devices SpectraMax Plus 384 Absorbance Reader Details. Microplate Readers. ) 45 (W) x 70 (H) x 27 (D) Dimensions (cm) 114 (W) x 178. for the SpectraMax Multi-Mode Microplate Reader, as well as all other Molecular Devices' microplate readers. 1536-well plates < 100 amol (384-well) 1 HTRF • Cisbio HTRF detection with optimized Xenon light source and . Supplier: Molecular Devices. The FLIPR 384 system comes complete with 384. The. 130009WThe SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. 0. For more sample throughput, the system canSpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384. As the wells of this plate are closer together, the light is more likely to stray into adjacent wells. UV absorbance detection. Mix well by trituration or plate shaker and incubate for 2 to 5 minutes at room temperature, protected from light. Molecular Devices: n/a:. Bioz Stars score: 86/100, based on 1 PubMed. x. 5577. The SpectraMax iD3 reader is built on the same foundation that has made our entire SpectraMax microplate reader product line among the most trusted in the. Documentation Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. the Molecular Devices SPECTRAmax® 190 and 250 plate. Visit the supplier page or submit a quote to learn more. In the M2, M5 and Plus. Multichannel processes: Costar 96-well (3300) and Greiner 384-well (781182/781185) microplates are validated for multichannel processes. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. #PLUS 384) UV-transparent 96-well microplates (Corning cat. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. The Standard Thermo Scientific 384-well plate, Barcoded, White, is compatible with the majority of 384 block PCR and sequencing instruments. Packed 25 plates per sleeve, 2 sleeves per case. SpectraMax® M2 and M2e Microplate Readers are the standard for UV/visible multimode reader absorbance, providing ultrafast and full spectral range detection for cuvettes and 6-, 12-, 24-, 48-, 96-, and 384-well microplates. 96- and 384-well plates; Wavelength(s): 635 nm (classification), 532 nm (reporter) Citations:. 00. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. Products : ClonePix 2 System, QPix 450/460 Systems, QPix 420 System, CloneSelect Imager. The guidelines for cuvette use in SpectraMax instruments are the same as those that apply to any high-quality spectrophotometer. Figure 1. 96-384. Each kit is sufficient for 3 Calcium 6 assay plates and 3 Calcium 6-QF assay plates. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Integrate any SpectraMax Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Microplates for applications that require high sample recovery rates. EMax. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. The SpectraMax Paradigm reader with HTRF Detection Cartridge offers high-throughput screening capability with excellent read times and Z’ factors. Analyst, M5, Flex 3, and SpectraMax L readers work differently. Monitor reader performance on demand to ensure data quality is not compromised between PM/OQ. The LMax plate reader has similar calculations and its RLUs are comparable to those of LMax II 384. Shaking: Linear, orbital, and double orbital. Growth medium was then replaced with fatty acid uptake solution. Fluorescent Polarization: Solid black plates, as above. The list is a standard grouping supplied by Molecular Devices and includes some which cannot be used on this instrument; they can be deleted. The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options. 20 attomol ATP per well. 11 10 100 1000 0 20 40 60 80 100 120 Calcium Mobilization in WT3M1 CHO Cells EC 50 = 13. 48 ± 0. FlexIII. Video Description: The video below gives an overview of the Molecular Devices SpectraMax M2 Multi-Mode Microplate Reader, and also demonstrates its functionality. Get Quote . Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. SLS & DLS: 2 µL quartz cuvettes 45 µL quartz cuvettes 4 mL generic glass cuvette DLS only:Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. 4 Equipment. explore more. A rinse program is included for quick and easy daily. B. Instrument pricing. Prepare human iPSC-derived neuronal progenitor cells (NPCs) according to Shi et al. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Tb. 5 ml microcentrifuge tubes 35 mm Petri dishes 12 mm × 50 mm test tubes (optional). In many cases these plates have the same dimensions, check the plate dimensions are the same as one of the plates listed above. Specialized filters allow for IR fluorescence detection ranging from 10-40 times more sensitive than conventional monochromator detection reader. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs), differentiated into. 100 fmol luciferase per well. The small-footprint Multiwash+ Microplate Washer quickly washes with both 96 and 384-well plates, has 20 standard wash protocols, and a simple LCD screen interface to customize speed, volume, soak time, and aspiration settings. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Supplier: Molecular Devices. For biochemical assays, the washer removes unbound material and unreacted reagents. Note: These settings are based on necessary cookies. M5. *For Corning UV plates, mean values at 260 nm ranged from 0. Gemini EM Reader scans. #6590-0144; includes Enhanced TRF Module, excitation and emission filters). Source plate capacity; 1 x plates. Align the two slots on the bottle holder with the two pegs on the rail. smartinject technology, atp a, spectramax i3x multi-mode microplate reader, luminescent cell vi, spectramax i3x multi mode microplate reader, spectramax i3x. Time-resolved phosphorescence. 0 out of 5 $ 14,499. x. 1. A rinse program is included for quick and easy daily. After 30 minutes the plate was read in Analyst GT with varying z heights. 2. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. (blue) and DsRed (red) transfected cells in 384-well plates, read from the bottom. We would like to show you a description here but the site won’t allow us. M5. 384 only) Selects either 96-well or 384-well plate for a stand-alone read. 057, depending on lot number. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader systems with all leading partner robots. plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. These lab workhorses are used in drug discovery, research, bioassay validation, and. Ensure your assay quality with quality tips Certified RNase-/DNase-free Nonpyrogenic Nonsterile 50 racks/case. Embla washers are durable and easy to maintain. Based on this customer experience and feedback, the Infinite 200 PRO microplate plate reader has been continuously engineered for. REF (SpectraMax Plus 384 only) A reading of buffer, water, or air taken in the cuvette that is used as I 0 to calculate Absorbance or % Transmittance. Microplate Spectrophotometer. #2704174, 2705402, 2705412, 2704180; Drummond Digital Microdispenser cat. Temperature Control: Ambient +5C up to 42C. x. Tecan Infinite 200 Pro M Plex Microplate Reader Unit2 - AV. 384-well TC plate, Poly-d-Lysine, Flat. The screw remains inside the instrument. 50 μL total well volume, with recommended working volume of 5-40 μL. TNF standard curve. Compare specifications and request a quote from leading suppliers. For more sample throughput, the system canThe SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. 8 mm distance between well bottoms to plate bottomValidated by Molecular Devices for the FLIPR reader. The SpectraMax® Plus 384 Microplate Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. x. 303 unable to cal dark current. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Category 2 and 3 microplate readers use gradient heating as well. Microarray scanners for one-fluor to multi-fluor [email protected] and Download Molecular Devices SPECTRAmax PLUS 384 operator's manual online. 2, prime the machine with CellTiter-Glo ® 2. 0 mP 384–1. This eliminates the need for standard curves, and Flexibility to read a variety of plate formats. 4 decades . using the upper dropdown menu to choose plate format (96 or 384 wells) and the "Select Specific" menu to choose the specific plate type. They are also fully compatible with the FlexStation 3 Multi-Mode Microplate Reader. Efficiency. 3. Molecular Devices has provided scientists with tools to expand the boundaries of their research. The FilterMax F5 can read 6, 12, 24, 48, 96, 384, 1536-well. 384-well TC plate, Poly-d-Lysine, Flat Clear. A snapshot of the Settings screen is shown in Figure 2. Learn more about SpectraMax Plus 384 Microplate Reader >>Rating: 5. Suggested plates are black. The third tested COC-based plate, the UltraVision 384-well microplate, however, absorbed at 2 OD at 200 nm,. #ABS Plus). Instrument settings are listed in Table 1. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. molecular interaction studies, and nucleic acid quantification, as well. Figure 2. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. 5–25 µl. the industry-leading microplate reader software for the SpectraMax Plus 384 system, allows complete customization of data collection and analysis. Well scans from the Gemini XPS Reader illustrating serial dilution of CHO cells using the Live Cell assay from Molecular Probes Live/Dead (Calcein AM /Ethidium Homodimer-1) assay kit. #3635) Transferpette micropipette (Drummond Scientific cat. For biotherapeutic development, the DynaPro Plate Reader is a comprehensive solution for stability evaluation. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as. Working in microplates allows for smaller assays that use less sample and reagent and increases throughput. Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. The plate reader consisted of a large-area. SoftMax® Pro Microplate Data Acquisition and Analysis Software Version 6 User Guide 5014177 C October 2011 FrameMaker_Export. xABS2, FL1, and LM1 Validation Plates sent to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, an then returned with a new certificate of calibration. Cross-talk <0. . Nanoracks’ Plate-Reader-2 (2nd generation) is a reconfigured Molecular Devices SpectraMax M5e – one of the most reliable, durable, feature-rich microplate readers on the market. Transfer the assay plate directly to a bottom-read fluorescence microplate reader for kinetic read-mode or incubate for 10-30 min at 37oC and then read for endpoint read-mode. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. For more sample throughput, the system canPlate Background OD, which was used for subsequent tests, was measured as follows. Outer wells aligned for proper viewing with FLIPR. FlexStation® 3 Multi-Mode MicroplateAlphaScreen and AlphaLisa detection using a 680 nm excitation laser diode and a 570/100 nm emission filter. It is more inert and reduces compound interference. The optical system of the SpectraMax 340PC384 Microplate Reader is built around a monochromator, which allows for selection of up to six wavelengths at a time for. Afterwards, 25 μL SpectraMax Glo Steady-Luc working solution was added to the wells. Determine concentration using the Beer-Lambert LawMolecular Devices’ FilterMax™ F5 Multi-Mode Microplate Reader was tested for compatibility with Life. #S1205. Includes PC and software. 7°C and the bottom to 36. Summary of Contents for Molecular Devices SpectraMax M2. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. based microplate readers that have 6well to 38 4well microplate reading capability as well as a builtin absorbance and fluorescence cuvette port. Filter 320/100 (available from Molecular Devices) Filter 616/10 (Molecular Devices cat. 0 OD for earlier models. Catalog number: AB1384W.